Figure 1
From: Differential Proteomics of Helicobacter pylori Associated with Autoimmune Atrophic Gastritis
Representative analytical DIGE proteome map of HP from AAG (A) and micro-preparative two-dimensional protein map of HP from AAG (B). (A) Proteins were resolved by IEF over the pl 3–10, followed by 8–16% gradient sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and overlaid by DeCyder. After extraction, proteins were labeled with Cy3 and Cy5. In particular, this gel (number 19) refers to samples P102cy3 (antrum) and P103cy5 (corpus) extracted from AAG-associated HP strains. An internal standard comprised of equal amounts of proteins from all samples (AAG, DU and GC) was labeled with Cy2 and included in all gels. (B) Gel number 18 is shown as representative of DIGE maps with proteins extracted from gastric cancer (cy5) comigrated with those extracted from H. pylori associated with DU (cy3). (C) Numbered spots indicate the differentially expressed proteins in AAG-associated HP strains in regards to either GC or DU. Identified spots are listed in Tables 2 and 3. Around 300 µg AAG-associated HP unlabeled protein pooled from amounts of samples was resolved by IEF over the pi range 3–10 NL, followed by 8–16% gradient SDS-PAGE and stained with CBB G-250. MW, Bio-Rad two-dimensional molecular weight standards.