Figure 4

Effects of CNDP2 on the cell apoptosis, cell cycle and ROS generation of gastric cancer cells. (A) The rate of cell apoptosis 48 h after AGS cells were transfected with CNDP2 was determined by flow cytometry. Statistical analysis of the percentages of the apoptotic cells. The experiments were performed in duplicate. (B) Cell cycle distribution was analyzed by FACS flow cytometry in AGS cells 48 h after they were transfected with GV142-CNDP2 or GV142-NC. The cell cycle distribution percentage shown is from three independent experiments. (C) AGS cells that were stably transfected with CNDP2 were loaded with DCFH-DA. The mean DCF fluorescence was measured. And the histograms are representative of the percentage of DCF fluorescence of three independent experiments. *p < 0.05, **p < 0.01, from the GV142-NC group. (D) The table reports the percentages of apoptotic SGC-7901 cells 48 h after they were transfected with Lesh-CNDP2 or Lesh-NC. (E) The cell cycle distribution was analyzed 48 h after SGC-7901 cells were transfected with Lesh-CNDP2 or Lesh-NC. The cell cycle distribution percentage shown is from three independent experiments.