Figure 2

α7 nAchR negatively regulates inflammasome activation. (A) LPS-primed peritoneal mouse macrophages from either WT or α7 nAchR knockout (a7 KO) mice were stimulated with ATP in the presence or the absence of acetylcholine for 30 min. (B) WT and a7 KO mice were injected intraperitoneally with LPS (10 mg/kg). Serum samples were collected 2.5 h later. (C) HEK293A cells were transfected with plasmids as indicated. At 24 h after transfection, cell lysates were harvested. IL-1 β cleavage and caspase-1 activation were assessed by Western blot analysis. Serum levels of IL-1 β, IL-18 or IL-6 were measured by ELISA. Blots are representative of three independent experiments. Data shown are means ± SD.