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Figure 1 | Molecular Medicine

Figure 1

From: Conditional Knockout of the RNA-Binding Protein HuR in CD4+ T Cells Reveals a Gene Dosage Effect on Cytokine Production

Figure 1

OX40-Cre HuRfl/+ and OX40-Cre HuRfl/fl knockout mice display significant decreases in HuR protein levels. (A) Western blot analysis of HuR expression (and β-tubulin as loading control) in activated T cells from OX40-Cre HuRfl/+ compared with HuRfl/+ control mice and (C) in activated T cells from OX40-Cre HuRfl/fl compared with HuRfl/fl control mice. (B) Intracellular HuR staining assessed by fluorescence-activated cell sorter analysis in activated T cells from OX40-Cre HuRfl/+ and HuRfl/+ control mice and (D) from OX40-Cre HuRfl/fl and HuRfl/fl control mice. (E) Proliferation assay reveals HuR knockout does not affect T-cell proliferation (mean ± standard error of the mean (SEM); n = 3). Western blot and intracellular HuR staining representative of n = 3.

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Molecular Medicine

ISSN: 1528-3658