Figure 2
Th2-polarized cells from OX40-Cre HuRfl/+ heterozygous knockout mice display decreases in Il4, Il13 and Gata3 steady-state mRNA levels. (A) Real-time PCR analysis of Gata3, II2, II4 and II13 mRNA in Th2-polarized cells. (B) Gata3 mRNA decay as assessed by real-time PCR in OX40-Cre HuRfl/+ and HuRfl/+ T cells (n = 3 for half-life). (C) ELISA of supernatants from Th2-polarized OX40-Cre HuRfl/+ mice. (D) Realtime PCR analysis of Ifng in Th1-polarized cells from OX40-Cre HuRfl/+ compared with HuRfl/+ control mice. (E) IFN-γ ELISA of supernatants from Th1-polarized OX40-Cre HuRfl/+ mice (error bars represent mean ± SEM; n = 3; *p < 0.05). Act. D, actinomycin D; N.S., not significant.