Figure 4
Il4 is transcriptionally upregulated in Th2-polarized cells from OX40-Cre HuRfl/fl homozygous knockout mice while Il13 and Il2 mRNA stabilities are increased. (A) Transcriptional assay using nascent RNA capture assay and real-time PCR analysis of Il13, Il4 and Il2 mRNA in Th2-polarized cells from OX40-Cre HuRfl/fl and HuRfl/fl mice. (B) Il2 mRNA decay as assessed by real-time PCR in OX40-Cre HuRfl/fl and HuRfl/fl T cells. (C) Il13 mRNA decay as assessed by real-time PCR in OX40-Cre HuRfl/f and HuRfl/f T cells. (D) Il4 mRNA decay as assessed by real-time PCR in OX40-Cre HuRfl/fl and HuRfl/fl T cells (error bars represent mean ± SEM; n = 3; *p < 0.05; n = 3 for half-life).