Figure 5
Polysomal distribution of Il2 and Il13 mRNA is unaltered in OX40-Cre HuRfl/fl homozygous knockout mice. (A) Absorbance profile for RNA separated by velocity sedimentation through a sucrose gradient. RNA was extracted from each fraction (B), and the levels of Il13 and Il2 mRNA (C) in each fraction from each population (OX40-Cre HuRfl/fl or HuRfl/fl control) was measured by RT-qPCR. (D) The levels of Gapdh mRNA were also measured in each fraction. The data shown are representative of two independent experiments. LMWP, low-molecular-weight polysomes; HMWP, heavy-molecular-weight polysomes.