Figure 1

Treatment of mice with 5-Aza inhibits the development of experimental autoimmune encephalomyelitis (EAE). (A) Low-dose 5-Aza (0.15 mg/kg/d) or control DMSO treatments of C57BL/6 mice were administrated at 5 d before induction of EAE for a total of 10 d. Each line represents an independent experiment. Two independent experiments were performed (n = 5 mice/group). The time of disease onset and peak is also indicated. (B) Spinal cords of mice treated with 5-Aza or DMSO were stained with H&E at the peak of EAE pathogenesis (upper panels: 40×, lower panels: 100×); arrows show inflammatory cell infiltration. Histology was performed in three different mice for each treatment, and representative images are shown. (C) Luxol fast blue stain of spinal cords at the peak of EAE in mice pretreated with 5-Aza or DMSO (40×). 5-Aza-treated tissue showed intact myelin, whereas DMSO-treated mice showed attacked myelin. Histology was performed in three different mice for each treatment. Representative images are shown.