Figure 2

5-Aza treatment decreases the number of CNS-infiltrating lymphocytes in EAE mice. Brains and spinal cords of MOG-induced EAE mice treated with 5-aza or DMSO were harvested at EAE disease onset or peak, and CNS-infiltrating lymphocytes were isolated by Percoll density gradient. Three independent experiments were performed. (A) Total infiltrating cell numbers were analyzed after Percoll gradient isolation. Open bars indicate 5-Aza treatment and black bars show control DMSO-treated mice (each group contained five mice, *p < 0.05). (B) CNS-infiltrating cells stained with CD3, CD4 and CD25, and CD3⁺ cells were gated to count CD4⁺CD25⁺ T cells from 5-Aza-treated (5-Aza, left panel) or DMSO-treated (DMSO, right panel) control EAE mice during disease onset (upper panel) and disease peak (lower panel). (C) Flow cytometry analysis of the expression of inflammatory cytokines (IFN-γ: upper panel; IL-17: lower panel) in CNS-infiltrating CD4⁺ T cells. A representative profile is shown. Three independent experiments were performed.