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Table 1 Assay of microparticles.

From: The Expression of HMGB1 on Microparticles Released during Cell Activation and Cell Death In Vitro and In Vivo

Forward and side light scattering

Staining with annexin V to detect phosphatidylserine

Staining for cell surface markers from cell of origin

Staining with STYO13 and other dyes binding nucleic acids

Functional effects in vitro (for example, immune activation, clotting)