Figure 2
From: The JAK-STAT Pathway Is Critical in Ventilator-Induced Diaphragm Dysfunction
JAK inhibition prevents the reduction of muscle-specific force that occurs in mechanically ventilated rats. (A) Mechanical ventilation induces STAT3 phosphorylation and JAK inhibitor blocks this activation. Control and MV (18 h) rat diaphragm muscles were subjected to Western blot analysis (n = 4 rats per group). (B-D) Treatment with a JAK inhibitor (R545) prevents MV-induced contractile dysfunction. The diaphragms from controls (n = 10) and MV rats treated with the JAK inhibitor (n = 8) or vehicle (n = 9) were analyzed: (B) Diaphragm strip ex vivo force-frequency relationship; (C) Specific force generation at 100 Hz; and (D) Fatigue development after 18 h of MV. Con, control.