Figure 3

Recombinant HSP25 induces myocardial NF-κB activation and IL-6 production and depresses cardiac function. Hearts from WT mice are perfused with recombinant heat HSP25 (0.25–2.0 µg/mL) for 30 min followed by a 70-min washout. Recombinant HSP25 increases myocardial NF-κB activity (expressed as fold-change above buffer perfusion control) and IL-6 levels and depresses cardiac contractility in a dose-dependent manner. Heat-denatured HSP25 at 2.0 µg/mL (2.0HD) fails to induce IL-6 production. Data are mean ± SE. n = 6; *P < 0.05 versus buffer perfusion control.