Figure 6

TLR2 KO and TLR4 mutation abrogates extracellular HSP27-induced NF-κB phosphorylation. MCVECs from TLR2 KO, TLR4-defective (C3H/HeJ) and WT mice were treated with 4.0 µg/mL HSP27 for 30 or 60 min. (A, B) Either TLR2 KO or TLR4 mutation abrogated NF-κB phosphorylation induced by extracellular HSP27. Normalized band density is presented as mean ± SE. n = 5; *P < 0.05 versus untreated control (ctrl); ^#P < 0.05 versus WT (C57 BL/6 or C3H/HeN).