Figure 4
(A,B) CXCR4/ACKR3 ligands regulate vasoconstriction upon α1-AR activation. Vasoconstriction was measured as change of the i.d. in pressure myography experiments. Mesenteric arteries were pressurized to 80 mmHg. *: p < 0.05 versus control (ctrl). Dose response to phenylephrine in the presence and absence of CXCR4/7 modulators (CXCL12, n = 4, ubiquitin (Ub), n = 5 [10 µmol/L) − 9 [1 µmol/L); TC14012, n = 9; AMD3100, n = 6; vehicle, n = 15). Phenylephrine and CXCR4/7 modulators were (A) added to the organ bath or (B) administered intraluminally. Vasoconstriction is expressed as % of i.d. in the absence of phenylephrine. (C) Mesenteric arteries were constricted with the EC50 dose of phenylephrine (1 µmol/L) and the effects of the CXCR4/7 modulators tested. The effects of the CXCR4/7 modulators (n = 4 to 7) are expressed as % change of i.d.