Figure 5
Blockade of CXCR4 with AMD3100 antagonizes effects of ubiquitin (Ub) and enhances effects of CXCL12 on phenylephrine-induced vasoconstriction. Mesenteric arteries were constricted with the EC50 dose of phenylephrine (1 µmol/L) and the effects of the CXCR4/7 modulators tested. The effects of the CXCR4/7 modulators are expressed as % change of i.d. *: p < 0.05 versus control (ctrl). (A) Dose response of ubiquitin and CXCL12 in the presence and absence of 50 µmol/L AMD3100; n = 4. (B,C) Addition of AMD3100 (50 µmol/L) (B) enhances CXCL12-mediated effects and (C) antagonizes ubiquitinmediated effects; n = 4.