Figure 6
ACKR3 ligands function as agonists with varying efficacy. Mesenteric arteries were constricted with the EC50 dose of phenylephrine (1 µmol/L) and the effects of the CXCR4/7 modulators tested. The effects of the CXCR4/7 modulators are expressed as % change of i.d. *: p < 0.05 versus control (ctrl). (A–C) Dose-dependent effects of (A) CXCL11, (B) CXCL12 and (C)TC14012 in the presence and absence of 100 nmol/L of the ACKR3 ligand CCX771 and CCX704 (control, inactive analogue of CCX771); n = 3 to 6. (D) Dose-dependent effects of the ACKR3 ligand CXC771; CCX704 was used as a control; n = 3. (E) Dose-dependent effects of the ACKR3 antibody 11G8; IgG1 was used as a control. n = 4. Ub: ubiquitin.