Figure 7
Effects of CXCR4/ACKR3 ligands on vascular α1-AR reactivity are specific. Vasoconstriction was measured as change of the i.d. in pressure myography experiments. *: p < 0.05 versus control. (A,B) Arteries were constricted to 40% to 60% of i.d. with (A) 60 mmol/L KCl (n = 3 to 4), (B) 800 pmol/L endothelin-1 (n = 3 to 6) and the effects of CXCR4/ACKR3 ligands (10 µmol/L) tested. The effects of the CXCR4/ACKR3 modulators are expressed as % change of i.d. (C) CXCR4/ACKR3 modulators do not affect the i.d. of mesenteric arteries at various transmural pressures; n = 4 to 8. Arrow: Addition of CXCR4/ACKR3 modulators, 10 µmol/L. (D) Mesenteric arteries pressurized to 30 mmHg were constricted with the EC50 dose of phenylephrine (1 µmol/L) and the effects of the CXCR4/ACKR3 modulators (10 µmol/L) tested; n = 3. (E) Mesenteric veins pressurized to 12 mmHg were constricted with the EC50 dose of phenylephrine (1 µmol/L) and the effects of the CXCR4/ACKR3 modulators (10 µmol/L) tested; n = 3 to 6.