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Figure 3 | Molecular Medicine

Figure 3

From: Adoptive Transfer of Heme Oxygenase-1 (HO-1)-Modified Macrophages Rescues the Nuclear Factor Erythroid 2-Related Factor (Nrf2) Antiinflammatory Phenotype in Liver Ischemia/Reperfusion Injury

Figure 3

Adoptive transfer of Ad-HO-1 BMMs upregulates Notch signaling and regulates IR inflammation in Nrf2-deficient livers (6 h of reperfusion after 90 min of ischemia). (A) Western blot analysis of hepatic HO-1, Notch1, Hes1, p-Stat3, total Stat3, p-Akt, total Akt and NF-κB expression in Nrf2 KO mice pretreated with Ad-HO-1/Ad-β-gal-transfected BMMs or untreated BMMs. Anti-β-actin antibody was used to assure equal protein amounts between samples. Data are representative of three experiments. (B, C) Quantitative real-time PCR-assisted detection of cytokine/chemokine programs in ischemic livers. *p < 0.05; **p < 0.005. Mean ± SD; n = 3–4/group.

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Molecular Medicine

ISSN: 1528-3658