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Figure 4 | Molecular Medicine

Figure 4

From: Adoptive Transfer of Heme Oxygenase-1 (HO-1)-Modified Macrophages Rescues the Nuclear Factor Erythroid 2-Related Factor (Nrf2) Antiinflammatory Phenotype in Liver Ischemia/Reperfusion Injury

Figure 4

Adoptive transfer of Ad-HO-1 BMMs ameliorates hepatocellular apoptosis in Nrf2-deficient mice (6 h of reperfusion after 90 min of ischemia). (A) Representative (n = 3) Western blot analysis of hepatic cleaved caspase-3, Bcl-2 and Bcl-xL after infusion of Ad-HO-1- or Ad-β-gal-transfected BMMs or untreated BMMs. (B) Representative (n = 3) TUNEL-assisted detection of apoptosis in ischemic liver tissue. Scale bars indicate 50 µm. (C) TUNEL+ cells were scored semiquantitatively by averaging the number of apoptotic cells (mean ± SD) per field. A minimum of 10 fields was evaluated/sample. *p < 0.001. There were 4–6 mice/group.

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Molecular Medicine

ISSN: 1528-3658