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Figure 5 | Molecular Medicine

Figure 5

From: Adoptive Transfer of Heme Oxygenase-1 (HO-1)-Modified Macrophages Rescues the Nuclear Factor Erythroid 2-Related Factor (Nrf2) Antiinflammatory Phenotype in Liver Ischemia/Reperfusion Injury

Figure 5

Nrf2-HO-1 axis promotes Notch1/Hes1/Stat3 signaling to regulate inflammatory response in vitro. Nrf2-deficient BMMs were transfected with Ad-HO-1 or Ad-β-gal (at an MOI of 10). After 36–48 h, cells were incubated with LPS (100 ng/mL) for 6 h. (A) Proteins isolated from Ad-HO-1/Ad-β-gal transfected or untreated BMMs were stained for HO-1, Notch1, Hes1, phos-Akt, phos-Stat3 and NF-κB. Anti-β-actin antibody was used to assure equal protein amounts between samples. Data are representative of three experiments. (B) Cytokine/chemokine programs analyzed by quantitative real-time PCR. *p < 0.05. Mean ± SD; n = 3–4/group.

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Molecular Medicine

ISSN: 1528-3658