Figure 5From: Reg3α Overexpression Protects Pancreatic β Cells from Cytokine-Induced Damage and Improves Islet Transplant OutcomeWestern blotting analysis of Reg3α effect on INS-1 cells viability and function. (A) After different treatment, Western blotting of Bcl-2, cleaved caspase-3 and total caspase-3 expression was showed and adjusted for tubulin expression. (B) Western blotting of Pdx-1, MafA, PCNA and p27Kip1 expression was performed. Tubulin was used as a loading control. A representative blot was presented from three independent experiments. (C–H) The ratio of interest protein to loading control was shown. Values represent the means ± SEM, n = 3. *P < 0.05 denoted significance.Back to article page