Figure 6

Reg3α overexpression suppressed cytokine-induced activation of p38 MAPK and ERK but not JNK phosphorylation. Vector or Reg3α-transfected cells and normal cultured cells were incubated in the presence/absence of the inflammatory cytokine cocktail from 0–120 min. The phosphorylation of p38 and ERK were detected by Western blotting. (A–C) A time course of p38 phosphorylation was indicated by Western blotting and adjusted for total p38 MAPK. The phosphorylated ratio of p38 MAPK adjusted for normal cultured group also was shown in different treated groups. (D–F) A time course of ERK phosphorylation was indicated by Western blotting and adjusted for total ERK. The phosphorylated ratio of ERK adjusted for normal cultured group was also shown. GAPDH was used as a loading control. A representative blot was presented from at least three independent experiments. Values represent the means ± SEM, n = 3. *P < 0.05 indicated significance.