Figure 6

PARP inhibition stimulates keratinocyte migration. Confluent cultures of keratinocytes were scratched with a pipette tip fixed to a liquid handling robot arm followed by washing and incubation of cells with medium (control), with PJ-34 (10 µmol/L) or 3-AB (3 mmol/L). Microphotographs were taken after 48 h. Representative images are presented (top) and repopulation of the scratched areas also was quantified by image analysis.