Figure 2

Histopathologic findings of spinal cords of the wild-type and Gnmt^−/− mice 17 d after immunization with MOG_35–55. (A–F) H&E sections; (G–L) sections stained with anti-CD45 antibody; (M–R), sections stained with Luxol fast blue (LFB). The micrographs of second, fourth and sixth row were the amplified images of the boxed areas in the first, third and fifth rows, respectively. As shown, the wild-type MOG_35–55 mice developed white matter damage with vacuoles on H&E sections (B,E) with CD-45 positive inflammatory infiltrates (H,K) and patchy pallor on LFB stain (N,Q), but the Gnmt^−/− MOG_35–55 showed scant or no damage in the corresponding areas. (S) Mean pathological scores of inflammation and demyelination ± SD are shown. Histology was performed in four different mice for each group, and representative images are shown. Scale bars in A–C, G–I, M–O: 500 µm; scale bars in D–F, J–L, P–R: 300 µm. Statistics were calculated by the Student t test. *p < 0.05.