Figure 4

GNMT deficiency suppressed MOG-specific T-cell responses in peripheral lymphoid tissue. (A) The recovered lymphocytes from draining LNs of 9-d-postimmunized mice were restimulated with various concentrations of MOG_35–55 peptide for 24 h. The proliferative response was determined by (³H)thymidine incorporation as described in Materials and Methods. (B and C) Lymphocytes were recovered from draining LNs of 9-d-postimmunized mice and stimulated with PMA and ionomycin in the presence of monensin. The percentages of Th1 and Th17-producing CD4⁺ T cells were analyzed by flow cytometry. Representative flow cytometric analysis (B) and the average percentages of CD4⁺ IFN-γ⁺ and CD4⁺ IL17A⁺ T cells (C). All data are presented as mean ± SEM from three mice in each group. Statistics were calculated by the Student t test. *p < 0.05; **p < 0.01.