Figure 6

Effects of GNMT deficiency on CD4⁺ T-cell differentiation in vitro. Purified CD4⁺CD25⁻ T cells from Gnmt^−/− and wild-type mice were stimulated with anti-CD3/CD28 (1 µg/mL) in Th1, Th17 or in Treg polarized conditions for 3 d. (A,C,E) Representative flow cytometric analysis and the histogram of percentage average of (B) CD4⁺ IFN-γ⁺, (D) CD4⁺ IL17A⁺ and (F) CD4⁺ Foxp3⁺ T cells. All data are presented as mean ± SEM. Statistics were calculated by the Student t test. **p < 0.01.