Figure 7

Ameliorated EAE in Gnmt^−/− mice was associated with higher amount of Treg cells. Lymphocytes were recovered from draining LNs of 9-d (initial phase)- or 18-d (effector phase)-postimmunized mice and stimulated with PMA and ionomycin in the presence of monensin. The percentages of CD4⁺ FoxP3⁺ T cells were analyzed by flow cytometry. (A,C) Representative flow cytometric analysis and (B,D) the average percentages of CD4⁺ FoxP3⁺ T cells in MOG_35–55-immunized wild-type and Gnmt^−/− mice at (A,B) initial or (C,D) effector phases. All data are presented as mean ± SEM. Statistics were calculated by the Student t test. *p < 0.05.