Figure 8

The efficiency of EAE induction of GNMT-deficient CD4⁺ T cells was lower than wild-type CD4⁺ T cells. (A and B) Wild-type and Gnmt^−/− mice were immunized with MOG_35–55 peptide/CFA. At d 9 postimmunization, mice were euthanized and total draining LN cells were further primed with 50 µg/mL MOG_35–55 peptide for 96 h. Then, the CD4⁺ T cells were purified (purity >95%) and transferred to naïve wild-type mice. (C and D) The CD4⁺ T cells (purity >95%) were isolated from wild-type and Gnmt^−/− mice and injected into Rag1^−/− recipient mice intravenously. After 24 h, Rag1^−/− recipients were immunized with MOG_35–55 peptide/CFA to elicit EAE. Data are shown as mean ± SEM for each group. Statistics were calculated by the Student t test. *p < 0.05.