Figure 2

Concomitance of sDF and CK in human spermatozoa. (A) Dot plots of the negative control (left panel) and the corresponding test sample (right panel) showing the percentages of sperm without sDF and CK (LL quadrant), sperm with sDF and without CK (UL quadrant), sperm with sDF and CK (UR quadrant) and sperm without sDF and with CK (LR quadrant). Representative of nine experiments. Percentages in the quadrants refer to the shown sample. (B) Percentage of CK in fragmented (UR/[UR + UL]) and nonfragmented (LR/[LL + LR]) sperm. (C) Images from fluorescence microscopy showing the localization of CK (left panel) in sperm with and without TUNEL labeling (middle panel). Right panel: bright field. Original magnification 1,000×. LL, lower left; UL, upper left; LR, lower right; UR, upper right.