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Figure 3 | Molecular Medicine

Figure 3

From: Investigation on the Origin of Sperm DNA Fragmentation: Role of Apoptosis, Immaturity and Oxidative Stress

Figure 3

Sorting fragmented and nonfragmented sperm by FACSAria II and staining with AB (n = 5). (A) TUNEL fluorescence histograms of the sorted fragmented and nonfragmented sperm. (B) Staining with AB of the sorted fractions. Sperm nuclei with intense dark-blue staining were considered to be immature. Original magnification 1,000×. (C) Percentage of chromatin immaturity, as detected by AB staining, in fragmented and nonfragmented sperm. **p < 0.01.

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Molecular Medicine

ISSN: 1528-3658