Figure 4

Concomitance of sDF and active caspases in human spermatozoa. (A) Dot plots of the negative control (left panel) and the corresponding test sample (right panel) showing the percentages of sperm without sDF and active caspases (LL quadrant), sperm with sDF and without active caspases (UL quadrant), sperm with sDF and caspase activity (UR quadrant) and sperm without sDF and with active caspases (LR quadrant). Representative of 13 experiments. Percentages in the quadrants refer to the shown sample. (B) Percentage of sperm with active caspases in fragmented (UR/[UR + UL]) and nonfragmented (LR/[LL + LR]) sperm. (C) Images from fluorescence microscopy showing the localization of active caspases (left panel) in sperm with and without TUNEL labeling (middle panel). Right panel: bright field. Original magnification 1,000×. ***p < 0.001. LL, lower left; UL, upper left; LR, lower right; UR, upper right.