Figure 5

Concomitance of sDF and cPARP in human spermatozoa. (A) Dot plots of the negative control (left panel) and the corresponding test sample (right panel) showing the percentages of sperm without sDF and cPARP (LL quadrant), sperm with sDF and without cPARP (UL quadrant), sperm with sDF and cPARP (UR quadrant), sperm without sDF and with cPARP (LR quadrant). Representative of 10 experiments. Percentages in the quadrants refer to the shown sample. (B) Percentage of sperm with cPARP in fragmented (UR/(UR + UL)) and nonfragmented (LR/(LL + LR)) sperm. (C) Images from fluorescence microscopy showing the localization of cPARP (left panel) in sperm with and without TUNEL labeling (middle panel). Right panel: bright field. Original magnification 1,000×. ***p < 0.001. LL, lower left; UL, upper left; LR, lower right; UR, upper right.