Figure 6

Concomitance of sDF and oxidative signs in human spermatozoa. 8-OHdG: (A) Dot plots of the negative control (left panel) and the corresponding test sample (right panel) showing the percentages of sperm without sDF and 8-OHdG (LL quadrant), sperm with sDF and without 8-OHdG (UL quadrant), sperm with sDF and 8-OHdG (UR quadrant) and sperm without sDF and with 8-OHdG (LR quadrant). Representative of 21 experiments. Percentages in the quadrants refer to the shown sample. (B) Percentage of sperm with 8-OHdG in fragmented (UR/[UR + UL]) and nonfragmented (LR/[LL + LR]) sperm, in total (left) and brighter (right) sperm population. MDA: (C) Dot plots of the negative control (left panel) and the corresponding test sample (right panel) showing the percentages of sperm without sDF and MDA (LL quadrant), sperm with sDF and without MDA (UL quadrant), sperm with sDF and MDA (UR quadrant) and sperm without sDF and with MDA (LR quadrant). Representative of 11 experiments. Percentages in the quadrants refer to the shown sample. (D) Percentage of sperm with MDA in fragmented sperm (UR/[UR + UL]) and nonfragmented (LR/[LL + LR]) sperm, in total (left) and brighter (right) sperm population. (E) Images from fluorescence microscopy showing the localization of MDA (left panel) in sperm with and without TUNEL labeling (middle panel). Right panel: bright field. Original magnification 1,000×. *p< 0.05; ***p < 0.001. LL, lower left; UL, upper left; LR, lower right; UR, upper right.