Figure 7
Experiments in live sperm. (A) Concomitance of sDF and 8-OHdG (n = 6). Dot plots of the negative control (upper panel) and the corresponding test sample (middle panel) showing percentage of: sperm without sDF and 8-OHdG (LL quadrant), sperm with sDF and without 8-OHdG (UL quadrant), sperm with sDF and 8-OHdG (UR quadrant), sperm without sDF and with 8-OHdG (LR quadrant). Representative of six experiments. Percentages in the quadrants refer to the shown sample. Lower panel, percentage of live sperm with 8-OHdG in fragmented (UR/(UR + UL)) and nonfragmented sperm (LR/(LL + LR)). (B) Concomitance of sDF and MDA (n = 6). Dot plots of the negative control (upper panel) and the corresponding test sample (middle panel) showing sperm without sDF and MDA (LL quadrant), sperm with sDF and without MDA (UL quadrant), sperm with sDF and MDA (UR quadrant) and sperm without sDF and with MDA (LR quadrant). Representative of six experiments. Percentages in the quadrants refer to the shown sample. Lower panel, percentage of live sperm with MDA in fragmented (UR/(UR + UL)) and nonfragmented sperm (LR/(LL + LR)). (C) Concomitance of sDF and active caspases (n = 7). Dot plots of the negative control (upper panel) and the corresponding test sample (middle panel) showing sperm without sDF and active caspases (LL quadrant), sperm with sDF and without active caspases (UL quadrant), sperm with sDF and active caspases (UR quadrant) and sperm without sDF and with active caspases (LR quadrant). Representative of six experiments. Percentages in the quadrants are referred to the shown sample. Lower panel, percentage of live sperm with active caspases in fragmented (UR/[UR + UL]) and nonfragmented sperm (LR/[LL + LR]). (D) Concomitance of 8-OHdG and active caspases (n = 3). Dot plots of the negative control (left panel) and the corresponding test sample (right panel) showing sperm without 8-OHdG and active caspases (LL quadrant), sperm with 8-OHdG and without active caspases (UL quadrant), sperm with 8-OHdG and active caspases (UR quadrant) and sperm without 8-OHdG and with active caspases (LR quadrant). Representative of three experiments. Percentages in the quadrants refer to the shown sample. *p < 0.05; **p < 0.01. LL, lower left; UL, upper left; LR, lower right; UR, upper right.