Figure 1
From: miR-145 Contributes to Hypertrophic Scarring of the Skin by Inducing Myofibroblast Activity
Fibroblast-to-myofibroblast differentiation in vitro. Skin fibroblasts were stimulated with TGF-β1 (2 ng/mL) for 24, 48 and 72 h. (A) Representative immunofluorescence pictures. Cells were stained with Phalloidin-Texas Red to label F-actin (red), DAPI to visualize the nucleus (blue) and anti-α-SMA antibody conjugated to Alexa Fluor 488 to visualize α-SMA (green) as a marker for myofibroblast differentiation. (B) α-SMA mRNA levels were assessed by real-time PCR and normalized to GAPDH mRNA levels. Graph depicts mean ± standard deviation (SD) (n = 4, **p < 0.01, ***p < 0.001). (C) Representative Western blot showing α-SMA protein levels; GAPDH was used as an internal control.