Figure 3
From: miR-145 Contributes to Hypertrophic Scarring of the Skin by Inducing Myofibroblast Activity
Inhibition of miR-145 expression decreases α-SMA expression in TGF-β1-stimulated skin myofibroblasts. (A–E) Primary skin fibroblasts were stimulated with TGF-β1 for 24 h and then treated with a specific miR-145 inhibitor for 48 h. A nonspecific Anti-miR was used as the control (mock). (A) miR-145 levels normalized to U6 snRNA levels. KLF4 (B) and α-SMA mRNA (C) levels normalized to GAPDH are shown. Representative Western blot (D) and densitometric analysis (E) of three Western blots showing α-SMA protein levels are shown. (F) Fibroblasts were stimulated with TGF-β1 for 24 h and then transduced with a KLF-4 encoding lentiviral vector. Nontransduced cells with and without TGF-β1 stimulation were used as controls. Graph depicts α-SMA mRNA levels normalized to GAPDH. Graphs show mean ± SD (n = 4, *p < 0.05, **p < 0.01, ***p < 0.001).