Figure 4
From: miR-145 Contributes to Hypertrophic Scarring of the Skin by Inducing Myofibroblast Activity
miR-145 inhibition abrogates contractile force generation in TGF-β1-induced skin myofibroblasts. Primary skin fibroblasts were stimulated with TGF-β1 for 24 h and then treated with a specific miR-145 inhibitor for 48 h. A nonspecific Anti-miR was used as the control (mock). Cells were then seeded into collagen gels, and gel contraction was assessed after 48 h. A representative picture of collagen gels (A) and densitometric analysis of gels from three independent experiments (B) are shown. Mean ± SD (***p < 0.001).