Figure 5
From: miR-145 Contributes to Hypertrophic Scarring of the Skin by Inducing Myofibroblast Activity
Inhibition of miR-145 decreases collagen 1A1 expression and TGF-β1 secretion by skin myofibroblasts. Skin fibroblasts were stimulated with TGF-β1 for 24 h and then treated with a specific miR-145 inhibitor. A nonspecific Anti-miR was used as the control (mock). At 48 h after transfection, cells were rinsed with PBS and analyzed by real-time PCR or incubated for another 48 h in growth medium without exogenous TGF-β1 for analysis of TGF-β1 secretion. (A) Collagen 1A1 mRNA level normalized to GAPDH. (B) TGF-β1 secretion level. Graphs show mean ± SD (n = 4, *p < 0.05).