Figure 1

Increased liver steatosis but decreased inflammation and fibrosis in LGALS3^−/− mice compared with WT mice on HFD. Representative histochemical and immunohistochemical staining of liver sections from WT and LGALS3^−/− mice on standard diet or HFD for 24 wks. (A) Gal-3+ cells (40×) and mRNA expression. (B) Oil red O staining (100×). Grading of liver steatosis. (C) H&E staining (40×). Grading of liver hepatocellular injury (hepatocyte ballooning) and inflammation. (D) Picrosirius red staining (10×). Grading of liver fibrosis. (E) Masson trichrome staining (10×). Hepatic mRNA expression for procollagen1A. (F) CD68+ cells (40×). (G) α-SMA+ cells (40×) and mRNA expression. The results are shown as the means ± SEM (n = 5–6 mice/group/experiment). *P< 0.05. The results are representative of two experiments.