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Figure 4 | Molecular Medicine

Figure 4

From: Galectin-3 Ablation Enhances Liver Steatosis, but Attenuates Inflammation and IL-33-Dependent Fibrosis in Obesogenic Mouse Model of Nonalcoholic Steatohepatitis

Figure 4

IL-33 expression in liver in mice on HFD. Effects of IL-33-stimulated macrophages in vitro. Representative immunohistochemical staining of liver sections and flow cytometric analysis of liver myeloid cells and IL-33 stimulated peritoneal macrophages. (A) IL-33+ cells (40×). (B) Hepatic mRNA expression of IL-33, ST2, IL-13 and TGF-β. (C) IL-13 expressing CD11b+ myeloid cells in livers of HFD or standard diet fed WT and LGALS3−/− mice for 24 wks. (D) Dose-dependent increase in the percentage of CD11b+ST2+ and CD11b+IL-13+ macrophages in response to in vitro stimulation with IL-33 (20),50 and 100 ng/mL). (E) IL-13 levels in supernatants. The results are shown as the means ± SEM (n = 5–6 mice/group/experiment). *P < 0.05, **P < 0.01. The results are representative of two experiments.

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Molecular Medicine

ISSN: 1528-3658