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Table 2 Hepatic mRNA expression levels of genes involved in lipid metabolism.a

From: Galectin-3 Ablation Enhances Liver Steatosis, but Attenuates Inflammation and IL-33-Dependent Fibrosis in Obesogenic Mouse Model of Nonalcoholic Steatohepatitis

 

WT CHOW

WT HFD

LGALS3−/− CHOW

LGALS3−/− HFD

ChREBP

1.08 ± 0.02

1.3 ± 0.23

1.27 ± 0.19

1.39 ± 0.19

LXR-α

1.02 ± 0.48

4.93 ± 0.95b

1.31 ± 0.66

1.65 ± 0.62#

LXR-β

0.93 ± 0.40

1.79 ± 0.3b

1.08 ± 0.27

1.42 ± 0.18

SREBP-1c

1.02 ± 0.23

1.76 ± 0.15b

0.89 ± 0.08

1.78 ± 0.23

PPAR-γ

1.03 ± 0.61

2.52 ± 0.53b

1.60 ± 0.50

5.57 ± 0.25b,c

Abca-1

0.32 ± 0.10

1.87 ± 0.57b

0.71 ± 0.30

4.87 ± 0.77b

Cd36

0.70 ± 0.43

1.20 ± 0.72

0.98 ± 0.56

4.70 ± 0.49b,c

FAS

0.66 ± 0.13

0.83 ± 0.23

0.42 ± 0.09

2.21 ± 0.31b,c

DAGT1

0.38 ± 0.12

1.64 ± 0.61b

0.33 ± 0.07

1.05 ± 0.23b

DAGT2

0.52 ± 0.22

3.15 ± 0.61b

0.51 ± 0.11

2.50 ± 0.26b

CPT1

0.51 ± 0.23

2.66 ± 0.87b

0.77 ± 0.27

2.67 ± 0.91b

  1. aRelative mRNA expression in liver was analyzed by qRT-PCR after 24 wks of HFD or standard diet feeding. The results are shown as the means ± SEM (n = 5–6 mice/group/experiment). The results are representative of two repeated experiments.
  2. bP < 0.05 versus CHOW-fed mice.
  3. cP < 0.05 versus WT mice.