Figure 1

LAIR-1 engagement by C1q or anti-LAIR-1 suppresses CpG-triggered production of cytokines in human monocytes. Purified human monocytes from healthy donors were unstimulated or stimulated with CpGODN 2216 (CpG), C1q with CpG or anti-LAIR-1 antibody with CpG for 4 h. Transcripts were determined by qRT-PCR. C1q or anti-LAIR-1 antibody inhibited CpG-mediated IFNα and IFNβ induction (A), IFN signature genes MX1 and OAS1 (B) and CpG-mediated IL-6 and TNFα induction (C). (D) After 24 h of culture, supernatants were collected, and the production of IL-6 and TNFα was measured by MSD assay. Utx, untreated. R.E., relative expression. Results represent the means ± standard error of the mean (SEM); the data were obtained from three independent experiments. *P < 0.05, **P < 0.01, ***P< 0.001.