Figure 2

C1q-meditated suppression of IFNα and TNFα production is reversed by LAIR-1 siRNA. (A, B) Human monocytes were transfected with control siRNA or LAIR-1 siRNA. After 24 h, cells were stimulated with CpG, C1q, CpG plus C1q or CpG and anti-LAIR-1 antibody for 4 h. Transcripts of IFNα and TNFα were determined by qRT-PCR. Results represent mean ± SEM; the data were obtained from three independent experiments. *P< 0.05, **P< 0.01, ***P < 0.0001, ns, not significant. Percent inhibition was calculated as based on qRT-PCR. (C) The efficiency of siRNA was determined at 24 h by flow cytometry with PE-labeled anti-LAIR-1 antibody.