Figure 2

Immunostimulatory properties of PG NSCs. (A) Cell proliferation in mixed lymphocyte reaction with irradiated autologous PBMCs (△), PG (□) or N (○) NSCs as stimulator cells and PBMCs as responder cells at a 1:1 stimulator-to-responder ratio. Proliferation was measured by BrdU incorporation and ELISA. For maximum stimulation, a PBMC sample was treated with ConA and PHA (stimulated auto, ▽). *p < 0.05 by Student t test. We analyzed three different donor samples (each sample was analyzed two to three times). Shown are the median for each condition. (B) Cytotoxicity assay. NKL effector cells were mixed with JEG-3 cells (◇), K562 (△), N (○) or PG NSCs (□) at 10:1 NKL-to-target ratios. NKL cell-mediated lysis was determined by lactate dehydrogenase release into the medium quantified by ELISA. **p < 0.001. n = 5.