Figure 7

HA-induced JAK3 activation stimulates Gai-mediated inhibition of AC, resulting in a decrease in the intracellular cAMP level 16HBE cells were pretreated with IBMX (1 mmol/L)for 15 min and then treated with forskolin (FSK, 10 µmol/L, 30 min) and either HA (40 µg/mL, 30 min) alone or HA (40 µg/mL, 30 min) in combination with IL-2 (100 UI/mL 30 min) as indicated. In some instances, cells were pretreated with pertussis toxin (PTx, 100 ng/mL) for 16 h or a TLR4 inhibitor (TLR4inh, 5 µmol/L) or JAK3 inhibitor VI (JAK3inh, 760 nmol/L) for 30 min. The intracellular cAMP levels were examined in the cell lysates. *p < 0.05 versus forskolintreated cells; ^#p < 0.05 versus HA-treated cells; and ^##p < 0.05 versus PTx-treated cells.