Table 1 Real-time PCR primer sequences.
Molecule | Sequence (5′–3′) |
|---|---|
IFN-γ forward | ACTGGCAAAAGGATGGTGAC |
IFN-γ reverse | TGAGCTCATTGAATGCTTGG |
TBX21 forward | ATTGGTTGGAGAGGAAGCGG |
TBX21 reverse | TGTGCACCCTTCAAACCCTT |
IL-18 forward | ATGCTTTCTGGACTCCTGCC |
IL-18 reverse | GTCTGGTCTGGGGTTCACTG |
IL-4 forward | ACGAGGTCACAGGAGAAGGGA |
IL-4 reverse | AGCCCTACAGACGAGCTCACTC |
GATA3 forward | GCTACGGTGCAGAGGTATCC |
GATA3 reverse | TTCACACACTCCCTGCCTTC |
IL-5 forward | CACCGAGCTCTGTTGACAAG |
IL-5 reverse | TCCTCGCCACACTTCTCTTT |
IL-33 forward | GGTCCCGCCTTGCAAAATAA |
IL-33 reverse | CCGTGGATAGGCAGAGAAGT |
IL-17 forward | TCCAGAAGGCCCTCAGACTA |
IL-17 reverse | AGCATCTTCTCGACCCTGAA |
RORγT forward | CTGTCCTGGGCTACCCTACT |
RORγT reverse | CCACTTGTTCCTGTTGCTGC |
IL-23 forward | AATAATGTGCCCCGTATCCA |
IL-23 reverse | CATGGGGCTATCAGGGAGTA |
TGF-β1 forward | TGCTTCAGCTCCACAGAGAA |
TGF-β1 reverse | TGGTTGTAGAGGGCAAGGAC |
IL-10 forward | ATAACTGCACCCACTTCCCA |
IL-10 reverse | GGGCATCACTTCTACCAGGT |
FOXP3 forward | CACCTATGCCACCCTTATCC |
FOXP3 reverse | CGAACATGCGAGTAAACCAA |
MMP-2 forward | CCTTGTTTACCATGGGTGGC |
MMP-2 reverse | AGCTCCTGGATCCCCTTGAT |
MMP-9 forward | AAAGGCAGCGTTAGCCAGAA |
MMP-9 reverse | ACAACTCGTCGTCGTCGAAA |
GAPDH forward | AACTTTGGCATTGTGGAAGG |
GAPDH reverse | CACATTGGGGGTAGGAACAC |