Figure 1

ADAMTS13 expression in inflammatory conditions. (A) ADAMTS13 transcript is decreased significantly in HSCs after stimulation with TNFα (100 ng/mL), IFNγ (100 ng/mL), IL-lβ (100 ng/mL), IL-2 (100 ng/mL), IL-6 (100 ng/mL) and pooled serum from septic patients (pooled SepSe) for 6 h or 24 h. Expression of ADAMTS13 based on Pfaffl method and normalized to reference transcripts (ADAM9 and HPRT). (B) ADAMTS13 transcript is significantly decreased in HMEC after stimulation with TNFα (100 ng/mL), IFNγ (100 ng/mL), IL-2 (100 ng/mL), IL-6 (100 ng/mL), cytokine mix (CM) and pooled SepSe for 6 h or 24 h. Expression of ADAMTS13 is normalized to reference genes (PBGD and HPRT). Bars represent mean values of log₂-ratios ± SEM for at least four independent experiments.