Figure 2

ADAMTS13 expression after stimulation with agents relevant for sepsis management. (A) ADAMTS13 transcript is decreased significantly in HSCs after stimulation with AVP and DDAVP (10 ng/mL), and activated protein C (APC, 10 ng/mL) for 6 h or 24 h. Expression of ADAMTS13 based Pfaffl method normalized to reference transcripts (ADAM9 and HPRT). (B) ADAMTS13 transcript in HMEC is altered after stimulation with AVP and DDAVP (10 ng/mL), with NE (10 ng/mL), APC (10 ng/mL) and selenite (1 ng/mL) for 6 h or 24 h. Expression of ADAMTS13 based on Pfaffl method and normalized to reference transcripts (PBGD and HPRT). Bars represent mean values of log₂-ratios ± SEM for at least four independent experiments.