Figure 3

High level of serum miR-150 is associated with higher tumor burden, treatment/death status and some prognostic markers. Serum miR-15 levels measured by qPCR were plotted with their median according to Binet stages (A), untreated/treated patients (B), alive/dead patients (C), β2-M (D), CD23 (E), LDT (F), IgHV mutational status (G), ZAP70 (H), LPL (I), CD38 (J) and cytogenetic abnormalities (K). MicroRNA expression was normalized with miR-16 expression and calibrated by subtracting 10 (chosen arbitrarily) from the ΔCt. The comparative ΔΔCq method was then applied for data analysis, and fold-changes were subsequently calculated (fold-change = 2^−ΔΔCt). Statistical differences were assessed by using the Kruskal-Wallis test for Binet stages, and the Mann-Whitney nonparametric test was used for other variables. Statistical details can be found in Supplementary Table S1.