Figure 6

The majority of serum miR-150 does not circulate in exosomes or microvesicles. A total of 15 CLL serum samples were centrifuged at 20,000g(to pellet microvesicles) and subsequently at 150,000g (to pellet exosomes). Uncentrifuged, vesicle-depleted and exosome-depleted sera were then compared. Cq values of miR-150 (A), miR-16 (B) and exogenous control cel-miR-39 (C) and cel-miR-59 (D) are provided. miR-150 (E) and miR-16 (F) Cq were normalized with the exogenous control cel-miR-39. The mean ratios in the two groups were plotted with their median. The Wilcoxon matched pairs nonparametric test was used to calculate significant differences.